Workshop Summary: Characterization of Fiber-based Scaffolds


At a 2013 ASTM workshop on measurements standards for scaffold it was determined that the major measurement needs were 1) structural, 2) mechanical and 3) biological (Simon et al. J Biomed Mater Res: Part B, 103B, 949-959, 2015). Fiber-based scaffolds are being widely advanced in tissue engineering for a wide variety of applications. The morning session of this workshop included talks on fiber-based scaffold characterization, batch to batch variability, measurement validation and release criteria.  In the afternoon, we held two Moderated Discussions that each focused on a particular measurement identified by the stakeholders as “in need of improvement”.  Also in the afternoon, there was also a computer-poster-networking-session.  The workshop concluded with a summary discussion and we considered the formation of working groups to address the issues identified, possibly through the ASTM standards development process.


Welcome: Opening Remarks, Becky Robinson-Zeigler (ARMI), Carl Simon (NIST)

Morning Talks:

  • Michael Yaszemski, Mayo Clinic: “Clinically Relevant Scaffold Specifications to Ensure Desirable Patient Outcomes” 

  • Alex Meltzer, DiPole Materials: “Standardized Scaffolds Through the Development of Relevant Specifications”         

  • Luca Cera, Harvard: “Rapid manufacturing of Biohybrid Fibrous Scaffolds for Biomimetic Heart Valve Replacement” 

  • Jayesh Doshi, eSpin: “Characterization of Fiber-Based Scaffolds”

  • Michael Francis, Embody LLC: “Clinical Translation of Engineered Microfibrous Collagen-Containing Grafts”

  • Seth McCullen, Poly-Med, Inc.: “Fiber-Based Scaffold Characterization for Medical Devices:

    Development, Implementation, and Utilization of Image-Based Test Methods”

Introduction to Breakouts:

Concurrent Breakout Sessions:

  • Measurement #1: Porosity (2 groups)

  • Measurement #2: Diffusivity (2 groups)

Networking Laptop “Poster” Sessions:

  • Chen Jiang, Kan Wang & Ben Wang (Georgia Institute of Technology): “Effect of Surface Morphologies on the Attachment of Mesenchymal Stem Cells (MSCs) to Electrohydrodynamic (EHD) Polycaprolactone (PCL) Fibers”

  • Chiara E. Ghezzi & David L. Kaplan (Tufts University): “Silk-Based Scaffolds for Tissue Engineering Applications”

  • Judith Heikoop, Ramon Solberg, Marc Simonet (IME Medical Electrospinning): “Limit Batch to Batch Variation by High Control on All Relevant Process Parameters”

  • Marley Dewey, Eileen Johnson, Daniel Weisgerber, Matthew Wheeler, Brendan A.C. Harley (University of Illinois at Urbana-Champaign): “Addition of Shape-fitting Poly(lactic Acid) Mechanical Supports and Immunomodulatory Amniotic Membrane to Enhance Mineralized Collagen Scaffolds for Craniofacial Bone Repair”

  • Ramon Solberg, Judith Heikoop, Marc Simonet (IME Medical Electrospinning): “Limit Batch Variation by Online Monitoring”

  • R. Glenn Hepfer, Peng Chen, Brooke J. Damon, Hai Yao (Clemson University): “Anisotropic Diffusivity Measured with Fluorescence Recovery after Photobleaching (FRAP)”

Report Outs:

Discussion and Wrap-Up: Workshop report, ASTM standards, formation of working groups (Carl Simon)


  • A report to summarize the workshop findings is being prepared for publication in the archival literature (contact Lexi Garcia,

  • A working group was formed to draft “ASTM Standard Guide for Characterizing Fiber-Based Constructs for Tissue Engineered Medical Products” (contact Carl Simon,


  • A working group was formed to discuss potential research projects to improve porosity measurements [(contacts Matei Cirstea ( & Carl Simon (]